The short answer is: it depends on the gift card.
We know that many users would like to print out their gift cards to use in a store or give as a physical gift. Unfortunately, it's tough for us to keep a running list of who accepts what and how, because, honestly, that varies so widely and changes so often. Some retailers do take them in the stores and some don't. It also changes seasonally, and even varies by location!
What our users have found to be the best approach in the past is to call the specific retail location you're planning to visit, ask to speak to the manager on duty, and ask them if their location takes gift cards that were purchased online. This way, you can get the answer straight from the horse's mouth, as they say, and also allows the store's staff to become familiar with their policies on e-gift cards if they're not already. It's win-win.